PODS® Human GDNF 胶质细胞源性神经营养因子
产品名称： PODS® Human GDNF 胶质细胞源性神经营养因子
英文名称： PODS® Human GDNF (Glial cell derived neurotrophic factor)
产品编号： PPH2
产品价格： 0
产品产地： 英国
品牌商标： Cell Guidance Systems
更新时间： null
使用范围： null
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PODS® Human GDNF
 |
 |
| PODS® co-crystals | PODS® co-crystals |
|
CellGS Catalogue Code |
Product Name |
Pack Size |
|
PPH2-50 |
PODS Human GDNF |
50 million |
|
PPH2-250 |
PODS Human GDNF |
250 million |
|
PPH2-1000 |
PODS Human GDNF |
1 billion |
PODS 技术
将生长因子包埋进蛋白质晶体（多角体蛋白）中，多角体蛋白能够保护包埋的生长因子，并维持其稳定性与生物活性，进而持续释放到细胞培养系统中。
PODS GDNF 描述
PODS多角体蛋白与GDNF共结晶。GDNF (Glial cell derived neurotrophic factor，胶质细胞源性神经营养因子)是由Lin等(1993)从鼠胶质细胞株B49的条件培养基中分离纯化获得的一种神经营养因子，且由此而命名。以纯化的GDNF的氨基端序列制作探针，克隆得到大鼠和人的GDNF基因。人GDNF前体蛋白为211个氨基酸残基(其中信号肽19个氨基酸)，加工处理后才形成分泌型的成熟蛋白，有134个氨基酸，是一个糖基化的二硫键连结的同源二聚体蛋白质，分子量为32~34kD，为碱性蛋白质。
The product contains the polyhedrin protein co-crystalized with Human GDNF. Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that signals through a multicomponent receptor system to activate receptor tyrosine kinase RET signaling. GDNF promotes dopamine uptake, prevents motor neuron apoptosis, and supports the survival and differentiation of neurons.
PODS GDNF 规格
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PODS GDNF Product Details |
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Length |
180 aa |
|
Structure |
Dimer |
|
Source |
Spodoptera frugiperda (Sf9) cell culture |
|
Accession Number |
P39905 |
|
Endotoxin Level |
<0.06 EU/ml as measured by gel clot LAL assay |
|
Formulation |
PODS® were lyophilized from a volatile solution |
|
Reconstitution |
PODS® co-crystals may be reconstituted at 200 million co-crystals/ml in water. 20% glucose has a buoyant density closer to PODS® co-crystals and can be useful for aliquoting. PODS® co-crystals are highly stable when stored in aqueous solution (pH range 6 - 8). |
|
Stability and Storage |
Upon receipt, store at 4°C. PODS® co-crystals are stable for at least 1 year when dry and 6 months when resuspended. |
PODS GDNF 使用建议
PODS® co-crystals provide a depot of proteins which are steadily secreted. It has been estimated that the biological activity of 50 million PODS® co-crystals generates the same peak dose as 3.3 μg of standard recombinant protein. However, at 5 days following the start of seeding the PODS® co-crystals, there are more than 50% of these peak levels still present in the culture system. Ultimately, the amount of PODS® co-crystals that is optimal for a particular experiment should be determined empirically. Based on previous data, we suggest using 50 million PODS® co-crystals in place of 3.3 μg of standard growth factor as a starting point."
To control for cross-reactivity with cells or as a negative control, we recommend using PODS® growth factors alongside PODS® Empty crystals, as the latter do not contain or release cargo protein.
PODS GDNF AA Sequence
MADVAGTSNR DFRGREQRLF NSEQYNYNNS KNSRPSTSLY KKAGLMSPDK QMAVLPRRER NRQAAAANPE NSRGKGRRGQ RGKNRGCVLT AIHLNVTDLG LGYETKEELI FRYCSGSCDA AETTYDKILK NLSRNRRLVS DKVGQACCRP IAFDDDLSFL DDNLVYHILR KHSAKRCGCI
PODS GDNF Alternative Names
Glial cell derived neurotrophic factor, ATF, HFB1-GDNF, ATF1, ATF-1, astrocyte-derived trophic factor 2, 胶质细胞源性神经营养因子
PODS GDNF 文件资料
 PPH2 Datasheet |
PODS User Guide |
PODS MSDS |
更多PODS 相关产品：PODS 生长因子
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