2x PCR Super Master Mix

Description

2 x PCR Super Master Mix is a premixed, ready-to-use solution containing DNA polymerases, dNTPs, MgCl2 and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR. The DNA polymerases are thermostable and possesse 3´→ 5´exonuclease activity, having a 6 fold increase in fidelity over routine Taq DNA Polymerase. The PCR products contain dA overhangs at the 3´– end and can be ligated to T/A vectors.

Components: 100 U/ml of Taq DNA polymerase, 0.5 mM dNTP, 4 mM MgCl2, 3´→ 5´ exonuclease, tracking dye and stabilizers.

Highlights

Robust and reliable reactions

All of the components are optimized, with the amplification efficiency exceeding 2kb/min.

High specificity and fidelity

The specially modified DNA polymerases ensure a PCR amplification with high specificity and fidelity.

Cost-effective

Cost-effective compared to our competitors.

Catalog #	B46015	B46018	B46019
2 x PCR Super Master Mix	500 rxns (5 mL)	2000 rxns (20 mL)	5000 rxns (50 mL)
User Guide	Yes	Yes	Yes

Storage

Product can be stored at -20 ℃ for 1 year, or stored at 4°C for up to 1 month.

Protocol

Make sure the 2 x PCR Super Master Mix is completely thawed, then mix well prior to use.

Add dd H2O, primers, template, and 2 x PCR Super Master Mix into a PCR tube according to the recommended concentrations. Give the mixture a quick spin in the centrifuge and load into PCR amplifier to begin amplification. We recommend assembling all reaction components on ice and quickly transferring the reactions to a thermocycler.

PCR Reaction Components	20 μL Reaction Volume (μL)	50 μL Reaction Volume (μL)
Forward Primer (10 μM)	0.5	1
Reverse Primer (10 μM)	0.5	1
Template	Variable	variable
2 x PCR Super Master Mix	10	25
ddH2O	to 20 μL	to 50 μL

PCR Steps	Temperature (℃)	Time	Cycles
1	94	5 min	1
2	94	20 sec	20-40
3	50-65	30 sec
4	72	X min (2 kb /min)
5	72	5 min	1
6	12	--	1

Trouble Shooting

Please review the following for trouble-shooting options when you encounter technical difficulties. Alternatively, feel free to contact Biotool technical support directly.

Problem	Potential Cause (s)	Suggestion (s)
No amplification product in test or control samples	Amplification reaction was incorrectly set up	Optimize the PCR reaction conditions
	Primers are not optimal	Redesign the primers
	PCR template was degraded	Collect fresh PCR template
Amplification worked in the control samples, but not in test samples	PCR Mix was stored too long and the activity was lost	Use a new PCR Mix
Non-specific amplification product (s)	Annealing temperature too low	Increase the annealing temperature
	The number of PCR cycles, primers concentration, or template concentration was too high	Decrease the number of cycles, primers concentration, or template concentration

更多详情请访问唯一官方网站http://www.biotool.cn/product/2x-pcr-super-master-mix.html

2x PCR Super Master Mix,中国库存，Biotool美国品牌。