BssNAI
产品名称： BssNAI
英文名称： BssNAI
产品编号： HZ600372
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
BssNAI
产品描述
使用说明
高浓度的酶活性，may result in to obtain 100%明星活动，牛血清白蛋白（BSA）should be added to the 1X混合反应到最后100μg／ml.do concentration of not use for long BSA的培养。
技术规格
Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时37°C in at总量在50μl反应。
网站gta↑tac recognition
嗜热脂肪芽孢杆菌的来源。
我们assayedλDNA
优化缓冲W（10 mM Tris HCl（pH 8.5（°C）；10 mM MgCl2 100毫米；NaCl；BSA DTT + 1毫米。）
37°C温度的优化
10毫米的存储条件的Tris-HCl（pH 7.5）；100 mM NaCl 0.1毫米；EDTA；7毫米2 -巯基乙醇；100μg／ml甘油50 %；BSA；商店在零下20摄氏度。
ligations after 10倍的酶overdigestion with more than 90% of DNA pBR322的碎片重新ligated and can be。
非特异性水解后没有检测到孵化nonspecific activity was withλDNA 1μg of of of hours for 16 20 UA听说是八周的酶在37°C。
甲基化效应不灵敏的测试
热灭活不
在campatible bst1107i，bstz17i，SNAI ^
使用说明
High enzyme concentration may result in star activity To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | GTA↑TAC |
| Source | Bacillus stearothermophilus NA |
| Assayed on | Lambda DNA |
| Optimal buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme more than 90% of DNA pBR322 fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | NO |
| Campatible Ends | Bst1107I, BstZ17I, SnaI^ |