官方网址：http://www.sinobiological.cn/CD48-SLAMF2-Antibody-g-7035.html
 CD48 / SLAMF2 Antibody Datasheet
 
 
 
 
 
 
 
 
 
Order or Inquire for CD48 / SLAMF2 Antibody product
 
 
 
 
 
 
 
Detection limit is 1 ng/lane in WB
 
 
 
 
 
 
 
 
 
 
CD48 / SLAMF2抗体产品信息
 
 
 
 
免疫原 : 
 
 
Recombinant Human CD48 / SLAMF2 Protein (Catalog#10797-H08H)
 
 
 
 
Antibody Type : 
 
Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
 
 
 
 
克隆号 :
 
 
 
009
 
 
 
 
抗体宿主 :
 
 
Rabbit IgG
 
 
 
 
缓冲液 :
 
0.2 μm filtered solution in PBS with 5% trehalose
 
 
 
制备方法 : 
 
 
This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human CD48 / SLAMF2 (rh CD48 / SLAMF2; Catalog#10797-H08H; NP_001769.2; Met 1-Ser 220).
 
 
 
 
 
CD48 / SLAMF2 Antibody Usage Guide
 
 
 
 
 
特异性 : 
 
 
 
Human CD48 / SLAMF2
 
 
 
 
 
 
No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
 
 
 
Western blot :
 
This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD48 in WB. Using a DAB detection system, the detection limit for Human CD48 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
 
 
 
储存方法 :
 
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
 Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
 
 
 
 
CD48 / SLAMF2 Antibody Related Products & Topics
 
 
Related Areas:
 
Proteins:
 
Antibodies:
 
 
CD48 / SLAMF2 Antibody Background
 
CD48, also known as SLAMF2, BCM-1 and BLAST-1, is a 65 kDa GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. In rodents, CD48 is the ligand for CD2 whereas in humans, CD58 is the ligand for CD2. Human CD48 cDNA encodes a 243 aa precursor that includes a 26 aa signal sequence, a 194 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide. It has a high degree of sequence homology to CD58 (LFA-3), and human CD48 shares approximately 50% aa sequence identity with mouse and rat CD48. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
 
参考文献
 
 
 
Wong, Y.W. et al., 1990, J. Exp. Med. 171: 2115-2130.
 
Ianelli,C.J. et al., 1997,J Immunol. 159 (8): 3910-3920.
 
Ianelli, C.J. et al., 1998, J. Biol. Chem. 273: 23367-23375.
 
Loertscher, R. et al., 2002, Transpl. Immunol. 9: 93-96.
 
Assarsson, E. et al., 2005, J. Immunol. 175: 2045-2049.
 
Bhat, R. et al., 2006, J. Leukocyte Biol. 79: 417-424.