Biotinylated Anti-Rat IL-4-抗体-抗体-生物在线
上海研卉生物科技有限公司
Biotinylated Anti-Rat IL-4

Biotinylated Anti-Rat IL-4

商家询价

产品名称: Biotinylated Anti-Rat IL-4

英文名称: Biotinylated Anti-Rat IL-4

产品编号: 500-P94Bt

产品价格: null

产品产地: USA

品牌商标: PEPROTECH

更新时间: null

使用范围: ELISA/WB

上海研卉生物科技有限公司
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产品编号:
500-P94Bt
来源:
Polyclonal Rabbit
制备:
Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IL-4. Anti-Rat IL-4 specific antibody was purified by affinity chromatography and then biotinylated.
免疫原:
E.coli derived Recombinant Rat IL-4 (PeproTech catalog #400-04)
应用:
ELISA
Direct
To detect Rat IL-4 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-4.
Sandwich
To detect Rat IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech’s Polyclonal Anti-Rat IL-4 (500-P94) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-4.
Western Blot
To detect Rat IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.