Assay principle

Orangu™ utilizes WST-8 which is metabolized by cells to give an orange-coloured formazan dye. The amount of dye formed is directly proportional to the number of living cells. Increasing incubation periods or number of cells will lead to an increase in the amount of orange dye. . The assay utilizes WST-8, a readily water-soluble tetrazolium salt, which, in the presence of an electron mediator, is reduced to an orange coloured formazan dye. Mitochondrial dehydrogenase enzymes within the cell act as an electron mediator such that the amount of the formazan dye generated is directly proportional to the number of viable cells and the length of incubation.

Simple Protocol

Product data

Citations

• Blum L, Geisslinger G, Parnham MJ, Grünweller A, Schiffmann S. (2020). Natural antiviral compound silvestrol modulates human monocyte-derived macrophages and dendritic cells. J Cell Mol Med DOI: 10.1111/jcmm.15360.

• Le-Trilling VTK, Becker T, Nachshon A, Stern-Ginossar N, Schöler L, Voigt S, Hengel H, Trilling M. (2020). The Human Cytomegalovirus pUL145 Isoforms Act as Viral DDB1-Cullin-Associated Factors to Instruct Host Protein Degradation to Impede Innate Immunity. Cell Reports 18;30(7):2248-2260.e5.

• Niknamian S. (2019). Fluoxetine can inhibit coxsackievirus-B4 E2 in vitro and in vivo. Antiviral Research 159:130-133.

• Benkahla MA, Alidjinou EK, Sane F, Desailloud R, Hober D. (2018). Fluoxetine can inhibit coxsackievirus-B4 E2 in vitro and in vivo. Antiviral Research 159:130-133. 

• Lutter AH, Scholka J, Richter H, Anderer U. (2017). Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures. Clinical Hemorheology and Microcirculation 67(3-4):327-342.