Dextrin Sepharose High Perform
产品名称： Dextrin Sepharose High Perform
英文名称： Dextrin Sepharose High Perform
产品编号： 28-9355-97
产品价格： 0
产品产地： 美国
品牌商标： GE
更新时间： null
使用范围： null
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- 邮编 : 201900
- 所在区域 : 上海
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| 28-9355-97 | Dextrin Sepharose High Perform 25ml |
| 28-9355-98 | Dextrin Sepharose High Perform 100ml |
| 28-9356-00 | StrepTactin Sepharose High Per |
| 28-9805-82 | MembraneProteinPurificationKit |
| 17-5468-15 | HiScreen Capto SP ImpRes |
| 17-5470-15 | HiScreen Capto Q ImpRes |
| 17-5474-15 | HiScreen MabSelect SuRe LX |
Dextrin Sepharose High Performance/MBPTrap HP
- Highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes
- MBPTrap HP 1 ml and 5 ml columns prepacked with Dextrin Sepharose™ High Performance give convenient, time-saving operation and reproducible results
- Physiological conditions and mild elution preserve the activity of the target protein
- Compatible with commonly used aqueous buffers and easily regenerated with 0.5 M NaOH
- Easy scale-up
- Convenient usage with a peristaltic pump, or chromatography system such as ÄKTA™ design
Dextrin Sepharose High Performance/MBPTrap HP
Technical Information
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Dextrin Sepharose High Performance and MBPTrap HP 1 ml and 5 ml columns for affinity purification of recombinant proteins tagged with maltose binding protein
Dextrin Sepharose High Performance and prepacked MBPTrap HP columns provide convenient affinity purification of recombinant proteins tagged with maltose binding protein (MBP). Dextrin Sepharose High Performance has high binding capacity for MBP-tagged proteins. This robust and stable Sepharose High Performance based medium enables elution of target protein in narrow peaks, which minimizes the need for further concentration steps.
Purification is performed under physiological conditions and the mild elution preserves the activity of the target protein. The mild conditions even allow purification of intact protein complexes. Due to the high specificity of the binding, very high purity of the eluted protein is achieved in just one step. Dextrin Sepharose High Performance and MBPTrap HP are easily regenerated using 0.5 M NaOH.
SDS-PAGE (reducing conditions) of the purification of MBP2*-paramyosin-d-Sal.
 and HiLoad 16/60 Superdex 200 pg (GF).'))
Automated purification of MBP2*-paramyosin-d-Sal using the two-step AC-GF protocol on MBPTrap HP 1 ml (AC) and HiLoad 16/60 Superdex 200 pg (GF).
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| TECHNICAL SPECIFICATIONS | |
| Matrix | Rigid, highly cross-linked 6% agarose |
| Average particle size | 34 μm |
| Ligand | Dextrin |
| Dynamic binding capacity1 | Approx. 7 mg MBP- DSal/ml medium |
| Approx. 16 mg MBP-bGal/ml medium | |
| Chemical stability | Stable in all commonly used aqueous buffers, 0.5 M NaOH (regeneration and cleaning) |
| pH stability | |
| working | > 7 |
| short term | 2¾13 |
| Storage | 4°C to 8°C in 20% ethanol |
| MBPTrap HP | |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1ml); 1.6 × 2.5 cm (5 ml) |
| Recommended flow rate2 | 1 ml/min (1 ml); 5 ml/min (5 ml) |
| Max. flow rate2 | 4 ml/min (1 ml); 20 ml/min (5 ml) |
| Max. pressure2 | 0.3 MPa, 3 bar |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 8°C |
| 1 Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-DSal (MBP2*-paramyosin-DSal, Mr approx. 70 000, multimer in solution), and MBP-bGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution) 2 H20 at room temperature |
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