比色法GAPDH分析试剂盒
产品名称： 比色法GAPDH分析试剂盒
英文名称： Colorimetric GAPDH Assay
产品编号： 8148
产品价格： 0
产品产地： 美国
品牌商标： sciencell
更新时间： 2024-10-28T10:24:25
使用范围： null
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比色法GAPDH分析试剂盒说明： 货号：8148
Description Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a tetrameric enzyme that catalyzes glycolysis and thus serves to break down glucose for energy and carbon molecules. Since it is ubiquitously expressed at relatively constant levels, GAPDH gene is widely used as an internal control for RNA and protein analysis. The ScienCell? Colorimetric GAPDH Assay provides a non-radioactive method to measure the enzyme activity of GAPDH in cultured cells, based on the oxidization of β-NADH to β-NAD in the presence of 3-phosphoglyceric acid (3-PGA), adenosine 5?-triphosphate (ATP) and GAPDH. The GAPDH activity is determined by assaying the rate of NADH oxidation, which is proportional to the reduction in absorbance at 340 nm over time (δA340nm/min).
This assay kit is used to evaluate GAPDH activity in vitro. It is for research use only. Not for use in animals, humans, or diagnostic procedures.
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