λDNA（dam-, dcm-）溶液（0.2 mg/ml）

附带条件

特技

λ是一种温带的大肠杆菌噬菌体。病毒DNA呈线性和双链（48502 NT），12 bp单链互补5’端。噬菌体颗粒将其染色体注入细胞后，染色体通过末端连接循环。在裂解途径中，表达编码复制、裂解和病毒蛋白的噬菌体基因。染色体复制，复制和裂解成子代噬菌体颗粒。在溶原途径中，噬菌体基因表达被抑制，环状染色体通过重组插入细菌染色体。

概述

Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5’-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis, and virion proteins are expressed. The chromosome replicates, and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination.