重组人LIF-RNAi技术-试剂-生物在线
上海沪震实业有限公司
重组人LIF

重组人LIF

商家询价

产品名称： 重组人LIF

英文名称： Recombinant Human Leukemia Inhibitory Factor

产品编号： HZ4488

产品价格： 0

产品产地： 中国/上海

品牌商标： 沪震生物

更新时间： 2023-08-17T10:24:20

使用范围： null

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 重组人LIF

中文名称：重组人LIF
英文名称：Recombinant Human Leukemia Inhibitory Factor
产品规格：5μg
分子量：Approximately 19.7kDa,a single non-glycosylated polypeptide chain containing 180 amino acids.
储存条件：冷冻（-20℃）

概述

Leukemia inhibitory factor(LIF)is a member of Interleukin 6 family.This protein is mainly expressed in the trophectoderm of the developing embryo,with its receptor LIFR expressed throughout the inner cell mass.LIF has the capacity to induce terminal differentiation in leukemic cells.Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells,the induction of neuronal cell differentiation,and the stimulation of acute-phase protein synthesis in hepatocytes.LIF is used in mouse embryonic stem cell culture,because that removal of LIF pushes stem cells toward differentiation,but they retain their proliferative potential or pluripotency.It is also used in phase II clinical trial,which can assist embryo implantation in women who have failed to become pregnant despite assisted reproductive technologies(ART).Mature human LIF(180 a.a.)shares 78%,82%,91%,88%and 87%a.a.sequence identity with mouse,rat,canine,bovine,and porcine LIF,respectively.

使用说明

This material is offered by Biorab Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.

技术规格
 

外观 Sterile Filtered White lyophilized(freeze-dried)powder.
纯度 ＞98%by SDS-PAGE and HPLC analyses.
来源 Escherichia coli.
生物活性 Fully biologically active when compared to standard.The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1ng/ml,corresponding to a specific activity of>1.0×107 IU/mg.
成分 Lyophilized from a 0.2μmfiltered concentrated solution in PBS,pH 7.4.
内毒素 Less than 1 EU/μg of rHuLIF as determined by LAL method.
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1%BSA to a concentration of 0.1-1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃.Further dilutions should be made in appropriate buffered solutions.
氨基酸序列 SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF

 

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！