Overview

Product name
Alkaline Phosphatase chromogen (BCIP/TNBT)

See all Alkaline Phosphatase Chromogen products (2) ...


Specificity
This product is a liquid substrate for alkaline phosphatase (AP), and is especially useful for the detection of AP-conjugated antibodies (i.e. secondary antibodies). Apply to tissue section for 10 minutes or until a suitable level of colouration has been achieved.


General notes
BCIP: 5-bromo-4-chloro-3-indolyl phosphate 0.452 mM TNBT: tetranitroblue tetrazolium 0.432 mM This product used to be called "Alkaline phosphatase detection kit (BCIP/TNBT

Properties

Storage instructions
Store at +4°C.


Storage buffer
pH: 9.20

Constituents: 0.02% BCIP, 0.04% TNBT


Concentration

 


    
    

    
Purity
    
Whole antiserum
    
    

    
IHC reagent notes
    
BCIP/TNBT is similar to BCIP/NBT but is more sensitive and produces a more intense purple color. A useful secondary marker in multiple staining procedures.
    
    

    
Research areas
    
Kits/ Lysates/ Other >> Tools and Reagents >> IHC Tools/ Reagents

     
    
    
http://www.abcam.com/Alkaline-Phosphatase-chromogen-BCIP-TNBT-ab7413.html