溶菌酶 0663
产品名称： 溶菌酶 0663
英文名称： Lysozyme
产品编号： 0663
产品价格： 0
产品产地： 北京市通州区马驹桥联东U谷86A
品牌商标： Solarbio
更新时间： 2023-08-11T10:26:26
使用范围： null
北京索莱宝科技有限公司
- 联系人 : 索莱宝-龚思雨
- 地址 : 北京市通州区中关村科技园区通州园金桥科技产业基地景盛南四街15号85A三层
- 邮编 : 101102
- 所在区域 : 北京
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溶菌酶
本产品用于生化研究，可水解革兰氏阳性菌和革兰氏阴性菌的细胞壁。
别名为胞壁质酶；Globulin G1；Muramidase
CAS号：12650-88-3
外观为白色冻干粉或结晶
R：42/43 S：36/37/39
-20℃ 条件下储存。
英文说明书：
LYSOZYME, EGG WHITE
Grade: ULTRA PURE GRADE
Appearance: White crystallized powder
Storage：Store frozen. Keep dry. Warm to room temperature before opening.
Product Description Lysozyme is a single chain polypeptide of 129 amino acids cross-linked with four disulfide bridges. Ithydrolyzes β(1→4) linkages between N-acetyl-muraminic acid and N-acetyl-D- glucosamine residues in peptidoglycan and between N-acetyl-D- glucosamine residues in chitodextrin.
The enzyme is often used for lysing bacterial cells by hydrolyzing the peptidoglycan present in the cell walls. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed more easily in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
Optimal pH: The activity of lysozyme is a function of both pH and ionic strength. The enzyme is active over a broad pH range (6.0–9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02–0.100 M) than at pH 9.2 (0.01–0.06 M).
Inhibitors:
Lysozyme is inhibited by indole derivatives, which bind to and distort the active site, and
imidazole, which induces the formation of a charge-transfer complex. It is also inhibited by surface-active agents such as sodium dodecyl sulfate, sodium dodecanate, and dodecyl alcohol. Other compounds of these types with carbon chains of 12 or more carbons in
length will also inhibit lysozyme.
Substrates:
The natural substrate for lysozyme is the peptidoglycan layer of bacterial cell walls.However, a variety of low molecular mass substrates including murein degradation products as well as synthetic compounds have been used for various photometric, isotopic, and immunological lysozyme assays.
Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses. Preparation Instructions For E. coli cell lysis, use a freshly prepared lysozyme solution (10 mg/ml) in 10 mM Tris-HCl, pH 8.0. The product is also soluble in water (10 mg/ml) yielding a clear to slightly hazy colorless solution. Aqueous solutions should retain activity for at least one month when stored between 2–8 °C. Storage/Stability
The product, as supplied, should be stored at –20 °C. When stored at –20 °C, the enzyme retains activity for at least 4 years. Solutions (pH 4–5) remain active for several weeks if refrigerated. Procedure The following procedure is for the lysis of E. coli. It may be used as a guideline for other species. The optimal pH for E. colicell lysis is 8.0±0.1.
1. Incubate E. coli strain bearing the pBR322 plasmid overnight with 25 μg/ml tetracycline and 25 μg/ml ampicillin.
2. Centrifuge 1–2 ml samples of the overnight culture.
3. Resuspend the pellets in 350 μl of STET buffer (10mM Tris-HCl, pH 8.0, with 0.1 M NaCl, 1mM EDTA, and 5% [w/v] TRITON X-100).
4. Add 25 μl of a freshly prepared lysozyme solution (10 mg/ml in 10 mM Tris-HCl, pH 8.0).
5. Mix by vortexing for 3 seconds.
6. Incubate the lysis mixture for 30 minutes at 37 °C
7. After incubation, place the tube containing the lysis mixture in a boiling water bath for exactly 40 seconds.
8. Centrifuge the lysis mixture at 14,000 g.
9. Remove the pellet (cell debris) from the tube using a sterile toothpick.
10.Plasmid DNA from the supernatant may then be purified and analyzed.
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