单羧酸转运蛋4抗体
产品名称： 单羧酸转运蛋4抗体
英文名称： MCT4/SLC16A3
产品编号： 2698
产品价格： null
产品产地： 上海
品牌商标： 雅吉
更新时间： null
使用范围： WB ELISA IHC-P IHC-F IF
上海雅吉生物科技有限公司
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| 中文名称 | 单羧酸转运蛋4抗体 |
| 别 名 | Monocarboxylate transporter 4; Slc16a3; Mct3; Mct4; MCT-4; Monocarboxylate transporter 3; solute carrier family 16 member 3; SLC16A3; MOT4_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 转录调节因子 转运蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, (predicted: Chicken, Dog, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 52kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC16A3:401-465/465 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 (SLC16A1; MIM 600682) and MCT2 (SLC16A7; MIM 603654), are characterized by 12 predicted transmembrane domains (Price et al., 1998 [PubMed 9425115]).[supplied by OMIM, Mar 2008] Function: Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Highly expressed in skeletal muscle. Similarity: Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. SWISS: O15427 Gene ID: 9123 Database links: Entrez Gene: 9123 Human Entrez Gene: 80879 Mouse Entrez Gene: 80878 Rat Omim: 603877 Human SwissProt: O15427 Human SwissProt: P57787 Mouse SwissProt: O35910 Rat Unigene: 500761 Human Unigene: 28632 Mouse Unigene: 10826 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Muscle (Mouse) Lysate at 30 ugPrimary: Anti-MCT4(bs-2698R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD  Sample: HepG2 Cell Lysate at 30 ugPrimary: Anti-MCT4(bs-2698R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD  Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCT4) Polyclonal Antibody, Unconjugated (bs-2698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MCT4 Polyclonal Antibody, Unconjugated(bs-2698R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |