PneumaCult™-ALI Medium
产品名称: PneumaCult™-ALI Medium
英文名称: PneumaCult™-ALI Medium
产品编号: 05001
产品价格: 0
产品产地: 加拿大
品牌商标: STEMCELL
更新时间: null
使用范围: null
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- 地址 : 浙江省杭州市西湖区申花路789号剑桥公社E座616室
- 邮编 : 310012
- 所在区域 : 浙江
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- 邮箱 : info@biowish.com
PneumaCult™-ALI is a serum- and BPE-free medium for the culture of primary human bronchial epithelial cells (HBECs) at the air-liquid interface (ALI). HBECs cultured in PneumaCult™-ALI undergo extensive mucociliary differentiation, resulting in cultures with morphological characteristics similar to that observed in the normal human airway. Cells cultured in PneumaCult™-ALI elicit physiological responses to known stimuli, making this medium a robust tool for in vitro airway epithelial cell research. PneumaCult™-ALI is also suitable for disease modeling, toxicity studies and preclinical drug development including inhaled drug formulations.
Application: Differentiation
Product Type: Specialized cell culture media
Recommended for: • Respiratory disease modeling
• Studying the development and maintenance of the respiratory epithelium
• Studying the response of the respiratory epithelium to internal or external stimuli
• Studying viral/bacterial infection
• Investigating the effects of inhaled toxins or particulate matter on the respiratory epithelium
• Preclinical drug development, including inhaled drug formulations
Components: • PneumaCult™-ALI Basal Medium, 450 mL (Catalog #05002)
• PneumaCult™-ALI 10X Supplement, 50 mL (Catalog #05003)
• PneumaCult™-ALI Induction Supplement, 1.8 mL (Catalog #05004)
• PneumaCult™-ALI Expansion Supplement, 1.8 mL (Catalog #05005)
• 4X PneumaCult™-ALI Maintenance Supplement, 1 mL (Catalog #05006)
Accessory Products: • Cell Culture Inserts for 12-Well Plates; 0.4 µm Transparent Polyester (BD Biosciences Catalog #353180)
• Heparin (Catalog #07980)
• Hydrocortisone (Catalog #07904)
• Trypsin-EDTA (Catalog #07910)
• Soybean Trypsin Inhibitor (Sigma Cat# T6522)
• Hanks Balanced Salt Solution without Ca++ and Mg++ (Catalog #37250)
• Collagen Solution (Catalog #04902)
Advantages: • HBECs cultured with PneumaCult™-ALI undergo extensive mucociliary differentiation to form a pseudostratified epithelium that closely resembles the human airway
• PneumaCult™-ALI is serum-free and BPE-free to minimize variability
Area of Interest: Pharmacology, toxicology, drug discovery, Respiratory research
Cell Type: Bronchial epithelial cells
Medium Type: Liquid, Serum-free
Popular Product Line: PneumaCult
Species: Human
Intended Use Statement: For Research Use Only. Not Intended for Human or Animal Diagnostic or Therapeutic Uses.
PneumaCult™-ALI是不含血清,不含BPE,用于人支气管上皮细胞(HBECs)在气 - 液界面(ALI)培养的培养基。 在PneumaCult™-ALI培养的HBECs可向纤毛进行分化,且其形态特征与正常人类呼吸道黏膜纤毛的分化类似。培养在PneumaCult™-ALI中的细胞具有生理环境类似的应激能力,因此该培养基在体外气道上皮细胞研究中非常有用。 PneumaCult™-ALI也适用于疾病模型,包括吸入药物制剂毒性研究和临床前药物开发。
应用范围:分化
产品类型:优化过的细胞培养基
推荐指数:呼吸系统疾病模型
•研究开发和维持呼吸道上皮细胞培养
•研究呼吸道上皮细胞内部或外部刺激响应
•研究病毒/细菌感染
•调查吸入毒素的影响或颗粒物对呼吸道上皮细胞的影响
临床前药物开发,包括吸入药物制剂
组成:•PneumaCult™-ALI基础培养基,450毫升(产品号#05002)
•PneumaCult™-ALI 10X添加物,50毫升(产品号#05003)
•PneumaCult™-ALI诱导添加物,1.8毫升(产品号#05004)
•PneumaCult™-ALI扩增添加物,1.8毫升(产品号#05005)
•4X PneumaCult™-ALI维持添加物,1毫升(产品号#05006)
配套产品:•12孔板细胞培养板,带0.4微米的透明聚酯的插入板(BD Biosciences公司产品编号353180)
•肝素(产品号#07980)
•氢化可的松(产品号#07904)
•胰蛋白酶-EDTA(产品号#07910)
大豆胰蛋白酶抑制剂(Sigma目录号#T6522)
•不含Ca++和Mg++的汉克斯平衡盐溶液(产品号#37250)
•胶原蛋白溶液(产品号#04902)
优点:•HBECs培养PneumaCult™-ALI进行黏膜纤毛的分化,形成假复层上皮,非常类似于人类呼吸道的结构
•PneumaCult™-ALI不含血清和BPE,可减少批间差异
领域:药理学,毒理学,药物发现,呼吸研究
细胞类型:支气管上皮细胞
培养基类型:液体,无血清
产品线:PneumaCult
种类:人
仅用于科研,不可用于临床