热休克转录因子2抗体
产品名称： 热休克转录因子2抗体
英文名称： HNF1A
产品编号： 1405
产品价格： null
产品产地： 上海
品牌商标： 雅吉
更新时间： null
使用范围： WB ELISA IHC-P IHC-F IF
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| 中文名称 | 热休克转录因子2抗体 |
| 别 名 | Heat shock factor protein 2; Heat shock transcription factor 2; HSF 2; HSF2; HSTF 2; HSF2_HUMAN. |
| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Rat, (predicted: Mouse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 59kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSTF2:401-536/536 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | The protein encoded by this gene belongs to the HSF family of transcription factors that bind specifically to the heat-shock promoter element and activate transcription. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]. Function: DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. Subunit: DNA-binding homotrimer in stressed or heat shocked cells, otherwise found as a homodimer. Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation. Similarity: Belongs to the HSF family. SWISS: Q03933 Gene ID: 3298 Database links: Entrez Gene: 3298 Human Entrez Gene: 15500 Mouse Omim: 140581 Human SwissProt: Q03933 Human SwissProt: P38533 Mouse Unigene: 158195 Human Unigene: 261395 Mouse Unigene: 447362 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 转录调节因子（Transcriptin Regulators） 热休克转录因子在热休克反应中的主要功能,是在热休克基因的表达过程中启动基因转录过程,促进热休克蛋白的表达. |
| 产品图片 |  Sample:HepG2 Cell(Human)Lysate at 30 ug Primary: Anti- HSTF2 (bs-1409R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59kD  Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(bs-1409R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. |