基本信息

产品名称FITC标记链霉亲和素英文名称Streptavidin protein (FITC)规格或纯度Streptavidin was conjugated with FITC under optimum conditions, and unconjugated Streptavidin and free FITC were removed.蛋白长度Full length proteinCarrier Free1% BSA偶联标记FITC. Ex: 493nm, Em: 528nm来源Native

相关属性

特点与好处Streptavidin (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.应用Flow Cytometry; IF;储存缓冲液PBS (pH 7.4) buffer containing 1% BSA and 0.05% ProClin™ 300 as a preservative.浓度Streptavidin Concentration: 1 mg/mL储存温度2-8°C储存,避免反复冻融运输条件冰袋运输稳定性与储存Shipped at 4℃. Store at 4°C and protected from prolonged exposure to light. Do not freeze.

常规信息

英文别名FITC Streptavidin; Streptavidin-FITC; FITC-streptavidin conjugate; SAv-FITC; Streptavidin Fluorescein Conjugated; Streptavidin-Fluorescein Isothiocyanate;别名FITC Streptavidin; Streptavidin-FITC; FITC-streptavidin conjugate; SAv-FITC; Streptavidin Fluorescein Conjugated; Streptavidin-Fluorescein Isothiocyanate; 链霉亲和素-FITC 来源于阿维丁链霉菌; 链霉亲和素-荧光素异硫*酸酯 (FITC) 偶联物; FITC链霉亲和素;

一般描述

背景

链霉亲和素（Streptavidin）是由streptomyces avidinii菌分泌的四聚体蛋白，可以高度特异性地和生物素（Biotin）结合，结合常数为10⁻¹⁴ mol/L，一分子 Streptavidin 可以结合 4 个 Biotin 分子，这种结合是目前自然界已知最强的非共价作用。 Streptavidin 和鸡蛋清来源的亲和素（Avidin）相比，两者与 Biotin 结合的亲和力高度相似性， 但Streptavidin在中性条件下几乎不带电荷，因此Streptavidin的非特异性结合比Avidin低很多， 检测时的非特异性背景也就少很多。Streptavidin 可以用于 Biotin 标记的抗体、核酸、蛋白或其他生物素标记分子的检测。Streptavidin (FITC)产品是用高纯度的 Streptavidin 和高纯度的 FITC 染料共价连接，并去除游离FITC。FITC标记的链霉亲和素是间接法荧光染色中一种非常有用的二抗，可以高亲和性地结合生物素化抗体。FITC由488nm的激光激发，可以在最大波长为520nm处检测到其发射光。

推荐工作浓度

Flow cytometry: 1:500-1:5000

IF: 1:50-1:500

Background

Streptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10⁻¹⁴ mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Unlike egg-white avidin, which has a net positive charge at neutral pH and contains about 7% carbohydrate, streptavidin has almost no net charge at neutral pH, does not contain carbohydrate, and exhibits lower non-specific background. Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules. This FITC-streptavidin conjugate was prepared by highly purified Streptavidin and free FITC was removed. Streptavidin (FITC) is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Excitation at 488nm light leads to a fluorescence emission maximum of 520 nm.

Recommended Usage：

Every lot of Streptavidin-FITC is tested by flow cytometry using biotinylated primary antibodies. From this testing it is recommended that between 0.02 and 0.25 µg of streptavidin be used per 106 cells in a 100 µl staining volume.

图片

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF-Streptavidin protein (FITC) (rp155876)

ICC/IF with Streptavidin protein (FITC) (rp155876) stained HeLa cells. The cells were methanol fixed (5 min) and blocked with 1% BSA . The cells were then incubated with Biotin-γ-actin antibody overnight at 4°C. Then Streptavidin protein (FITC) rp155876 (green) was used at 1:1000 for 1h. DAPI was used to stain the cell nuclei (blue) .

Flow-Streptavidin protein (FITC) (rp155876)

Flow-Streptavidin protein (FITC) (rp155876)

BALB/c splenocytes stained with Biotin-CD45R antibody, followed by Streptavidin protein (FITC) (rp155876) at 1/2500 dilution.