AcuI
产品名称： AcuI
英文名称： AcuI
产品编号： HZ600562
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
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AcuI
产品描述
使用说明
高浓度may result in星的酶活性。to obtain 100% activity，BSA should be added to the 1X混合反应到最后100μg／ml浓度和山姆should be 0.01毫米的浓度添加到最后吗？do not use for long BSA的培养。
技术规格
Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时37°C in at总量在50μl反应。
ctgaag 16↑识别网站（N）
应变检测大肠杆菌的来源，从ACU carries the I基因srw4鲍曼不动杆菌
我们assayedλDNA
优化的缓冲区（33毫米和醋酸（pH 7.9毫米（10°C）；醋酸醋酸钾镁；66毫米1毫米；BSA DTT + +山姆。）
37°C温度的优化
10毫米的存储条件的Tris-HCl（pH 7.5）；100 mM NaCl 0.1毫米1毫米；EDTA；BSA DTT；200μg／ml甘油50 %；商店；在零下20摄氏度。
ligations after about with 2倍的酶overdigestion 80% ligated can be of the DN**段。can be of these，收到了80%。
非特异性水解后没有检测到nonspecific孵化of activity was with 2λDNA 1μg of 16 hours of UA听说是八周的酶在37°C。
甲基化效应不灵敏的测试
热灭活的灭活酶是由孵化在150度为20分钟。
campatible eco57i。
使用说明
High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml and SAM should be added to a final concentration 0.01 mM? Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | CTGAAG(N)16↑ |
| Source | E.coli strain that carries the cloned Acu I gene from Acinetobacter calcoaceticus SRW4 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA + SAM |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C. |
| Ligations | After 2-fold overdigestion with enzyme about 80% of the DNA fragments can be ligated. Of these, 80% can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65°C for 20 mins. |
| Campatible Ends | Eco57I |