间充质干细胞培养基MSCM-细胞/细菌培养-试剂-生物在线

产品名称：间充质干细胞培养基MSCM

英文名称： MSCM

产品编号： 7501

产品价格： 0

产品产地：美国

品牌商标： ScienCell

更新时间： null

使用范围： null

北京裕恒丰科技有限公司

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Product Description

Mesenchymal Stem Cell Medium (MSCM), when used with Mesenchymal Stem Cell Growth

Supplement (MSCGS, Cat #7552) and 25 ml of fetal bovine serum (FBS) is a complete medium

designed for optimal growth of normal mesenchymal stem cells in vitro. It is a sterile, liquid

medium which contains essential and non-essential amino acids, vitamins, organic and inorganic

compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine

serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when

equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated

(quantitatively and qualitatively) to provide an optimally balanced nutritional environment that

selectively supports the growth of normal mesenchymal stem cells in vitro.

Components

MSCM consists of 500 ml of basal medium, 25 ml of FBS (Cat. #0025), 5 ml of Mesenchymal

Stem Cell Growth Supplement (MSCGS, Cat. #7552) and 5 ml of penicillin/streptomycin

solution (P/S, Cat. #0503). Note: FBS, MSCGS and P/S are not pre-mixed in MSCM; they must

be added separately to make the complete MSCM.

Product Use

MSCM is for research use only. It is not approved for human or animal use, or for application in

in vitro diagnostic procedures.

Storage

Store the basal medium at 4o C and the MSCGS, FBS and P/S solution at -20o C. Protect from

light.

Shipping

Basal medium: room temperature. Supplements: dry ice.

Instructions for Use

Thaw MSCGS, FBS and P/S solution at 37o C. Gently tilt the tubes several times to ensure the

contents are completely mixed before adding to the medium. Spray the medium bottle and tubes

with 70% ethanol, and wipe to remove excess liquid. In a sterile field, remove the caps without

touching the interior threads with fingers. Add MSCGS, FBS and P/S solution to the medium

and mix well. Since several components are light-labile, the medium should not be exposed to

light for extended periods. We do not recommend warming medium in a 37o C water bath prior to

use. When stored in the dark at 4o C, the reconstituted medium is stable for one month.

Caution: If handled improperly, some components of the medium may present a health hazard. Take appropriate

precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly