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Method of Production  : Transient transfection in 293 cell culture    

Method of Purification  : Protein A affinity chromatography and SEC

Formulation:   PBS pH7.4

Concentration:   2.50 mg/mL

Eedotoxin：＜1EU/ug

0.2μm Filtration：Yes

表达宿主：Human Cells

纯度:> 95 % as determined by SDS-PAGE.

内毒素：<1.0 EU per μg protein as determined by the LAL method.

蛋白构建：

A DNA sequence encoding the receptor binding domain (RBD，319-541aa) of 2019-nCoV spike was expressed with the Fc region of mouse IgG1 at the C-terminus.

GIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTN

融合Fc标签氨基酸序列：

PRDCGCKPCICTVPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVEVHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIEKTISKTKGRPKAPQV

YTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQWNGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLHNHHTEKSLSHSPGK

分子量：

The recombinant receptor binding domain (RBD) of 2019-nCoV spike consists of 457 amino acids and predicts a molecular mass of 51.5 kDa.