Description Invent Biotechnologies MinuteTM chloroplast isolation kit is composed of optimized chloroplast isolation buffers and filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate intact chloroplasts from fresh plant tissues (leaves, seeds and soft stems etc.). Due to the use of filter cartridges with pre-defined pore size and thickness, intact chloroplasts can be isolated from 5-200 mg fresh plant tissues in less than 5 min. Unlike many other methods that require 1-10+ gram tissues for chloroplast isolation, this kit can quickly obtain 1 X 106 to 1 X 107 intact chloroplasts (>90% intact) from fresh plant leaves.

Application MinuteTM chloroplast isolation kit is designed to rapidly isolate intact chloroplasts from small samples (5-200 mg) of fresh plant tissues for applications such as biochemical analysis, SDS-PAGE, immunoblottings, enzyme assays etc. Isolated chloroplasts are ideal starting materials for purification of RNA and DNA for molecular biology studies.

Buffer Formulation: Proprietary

Kit components 1. 25 ml buffer A 2. 25 ml buffer B 3. 50 protein extraction filter cartridges 4. 50 collection tubes with cap 5. Plastic rod (4)
Storage: Store buffer A and B at -20oC

Additional Materials Required Table-Top Microcentrifuge 1 X PBS or 1 X TES buffer