HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs

• Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
• Sephacryl™ S-100 HR is designed for separating peptides and small proteins.
• S-200 HR and S-300 HR are for purifying antibodies, serum proteins, and mid-size proteins.
• S-400 HR and S-500 HR are used to separate polysaccharides, macromolecules with extended structures, and even small plasmids.
• S-1000 SF is optimized for the purification of dextrans up to 10⁸ molecular weight, spherical particles up to 400 nm, and DNA up to 20 000 base pairs including plasmids, vesicles, and viruses.
• Convenient, prepacked HiPrep™ Sephacryl™ columns speed up protein purification.

• HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs

  Technical Information

  
  
  HiPrep™ Sephacryl™ HR columns, prepacked with Sephacryl™ High Resolution media, provide reliable gel filtration separations.

   

  HiPrep™ Sephacryl™ HR prepacked gel filtration columns offer excellent separation properties in a convenient, ready-to-use format. By combining the performance of Sephacryl™ High Resolution media with a low cost column construction, they are both reliable and cost effective to use with simple equipment or systems.

   

  Five HiPrep™ Sephacryl™ HR columns are available prepacked with S-100, S-200, S-300, S-400, or S-500 HR media, respectively. Each column is also available in two sizes: 16/60 for sample volumes of up to 5 ml and 26/60 for sample volumes of up to 13 ml. The range of HiPrep™ Sephacryl™ HR prepacked columns allows purification of molecules with molecular weights as small as 1 × 10³ to macromolecules as large as 2 × 10⁷.

   

  TECHNICAL SPECIFICATIONS
  Sephacryl™ High Resolution
  Fractionation range (Mr) -
  - Globular proteins
  S-100 HR   S-200 HR   S-300 HR   S-400 HR	1 × 103 - 1 × 105 5 × 103 - 2.5 × 105 1 × 104 - 1.5 × 106 2 × 104 - 8 × 106
  - Dextrans
  S-200 HR   S-300 HR   S-400 HR   S-500 HR   S-1000 HR	1 × 103 - 8 × 104 2 × 103 - 4 × 105 1 × 104 - 2 × 106 4 × 104 - 2 × 107 5 × 105 - > 108
  Exclusion limit up to 400 nm for spherical particles.
  - DNA exclusion limit
  S-200 HR   S-300 HP   S-400 HR   S-500 HR   S-1000 HR	30 bp 118 bp 271 bp 1078 bp 20 000 bp
  Matrix	Spherical allyl dextran and N, N’-methylenebisacrylamide
  Chemical stability	Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine-HCl, 1% SDS, 2 M NaCl, 24% ethanol, 30% propanol, 30% acetonitrile, 0.5 M NaOH (for cleaning)
  pH stability	3-11 (long term and working) 2-13 (short term)
  Storage	20% ethanol
  Storage temperature	4°C to 30°C
  Physical stability   Autoclavability   Antimicrobial agent	Negligible volume variation due to changes in pH or ionic strength   At 121°C, pH 7 for 30 minutes (gel only)   20% ethanol
  Autoclavability	At 121°C, pH 7 for 30 minutes (gel only)
  Autoclavability	At 121°C, pH 7 for 30 minutes (gel only)
  Antimicrobial agent	20% ethanol
  Autoclavability	At 121°C, pH 7 for 30 minutes (gel only)
  Antimicrobial agent	20% ethanol
  Autoclavability	At 121°C, pH 7 for 30 minutes (gel only)
  Antimicrobial agent	20% ethanol

   

  
  Selectivity of the five different prepacked HiPrep Sephacryl™ HR columns.

   

  TECHNICAL SPECIFICATIONS
  Prepacked HiPrep™ Sephacryl HR Columns
  Bed dimensions
  HiPrep™ 16/60   HiPrep™ 26/60	16 × 600 mm 26 × 600 mm
  Bed volume
  HiPrep™ 16/60   HiPrep™ 26/60	120 ml 320 ml
  Sample volume
  HiPrep™ 16/60   HiPrep™ 26/60	< 5 ml < 13 ml
  Rec. linear flow rate	5-15 cm/h
  Max. linear flow rate	30 cm/h
  (H2O at 25°C)
  Max. pressure over the packed bed during operation	1.5 bar (22 psi, 0.15 MPa)
  Column hardware pressure limit	5 bar (73 psi, 0.5 MPa)
  Theoretical plates	5000 m-1
  Storage	20% ethanol
  Storage temperature	4°C to 30°C