PvuII
产品名称： PvuII
英文名称： PvuII
产品编号： HZ600222
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
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- 邮编 : 200612
- 所在区域 : 上海
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PvuII
产品描述
使用说明
高浓度may result in星的酶活性。to obtain 100% activity，BSA should be added to the反应混合到最后1 100μg／ml浓度的BSA。do not use for long孵化。
技术规格
Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时37°C in at总量在50μl反应。
网站cag↑ctg recognition
应变检测大肠杆菌的来源，from the基因PvuⅡ带变形杆菌
我们assayedλDNA
优化缓冲G？（10mM Tris-HCl（pH 7.6毫米（°C）；10 50毫米氯化钠；氯化镁；1毫米DTT。）+？牛血清白蛋白（BSA）
37°C温度的优化
10毫米的存储条件的Tris-HCl（pH 7.5）；300 mM NaCl 0.1毫米；EDTA；7毫米2 -巯基乙醇100μg／ml；BSA；50%的甘油。商店在零下20摄氏度。
ligations after 10倍overdigestion with more than 70 %的酶的DNA碎片重新ligated and can be。
非特异性水解后没有检测到孵化nonspecific activity was withλDNA 1μg of of of 10 16 hours for UA听说是八周的酶在37°C。
甲基化效应不灵敏的测试
热灭活的酶灭活°C is at by潜伏期为20分钟。
使用说明
High enzyme concentration may result in star activity. To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.?
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Recognition sites | CAG↑CTG |
| Source | E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris |
| Assayed on | Lambda DNA |
| Optimal buffer | G?(10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) +?BSA |
| Optimal temperature | 37°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme more than 70% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |