别    名Interleukin-1 receptor antagonist; ICIL 1RA; IL1 inhibitor; ICIL1RA; IL1F3; IL1RN; IRAP; IL1RA_HUMAN.  

研究领域免疫学  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Sheep, )

产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量17kDa

细胞定位细胞浆 分泌型蛋白 

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human IL-1RA:26-120/178 

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

 

Function:

Inhibits the activity of interleukin-1 by binding to eceptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.

 

Subcellular Location:

Isoform 1: Secreted.

Isoform 2, 3, 4: Cytoplasm.

 

Tissue Specificity:

The intracellular form of IL1RN is predominantly expressed in epithelial cells.

 

DISEASE:

Microvascular complications of diabetes 4 (MVCD4) [MIM:612628]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.

Interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]: A rare autoinflammatory disease of skin and bone resulting in sterile multifocal osteomyelitis, periostitis, and pustulosis from birth. The term autoinflammatory disease describes a group of disorders characterized by attacks of seemingly unprovoked inflammation without significant levels of autoantibodies and autoreactive T-cells. Note=The disease is caused by mutations affecting the gene represented in this entry.

 

SWISS:

P25085

 

Gene ID:

3557

 

Database links:

Entrez Gene: 3557 Human

 

Entrez Gene: 16181 Mouse

 

Entrez Gene: 60582 Rat

 

Omim: 147679 Human

 

SwissProt: P18510 Human

 

SwissProt: P25085 Mouse

 

SwissProt: P25086 Rat

 

Unigene: 81134 Human

 

Unigene: 882 Mouse

 

Unigene: 162640 Rat

 

 

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

 

IL-1受体拮抗剂(IL-1ra)是IL-1强有力的内生拮抗剂，通过与白细胞介素-1的Ⅰ型和Ⅱ型受体结合，特异性地阻断白细胞介素-1的生物学作用。白介素1受体拮抗剂(IL-1Ra)是体内天然的一种对IL-1有拮抗作用的细胞因子。

产品图片

Sample: Lane1:Plasma(Mouse) Tissue Lysate at 30 ug Lane2:Hemocyte(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (bs-2216R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kD Sample: Spleen(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (bs-2216R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kD Paraformaldehyde-fixed, paraffin embedded (mouse spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1RA) Polyclonal Antibody, Unconjugated (bs-2216R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: Human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-1RA Polyclonal Antibody, Unconjugated(bs-2216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining