Overview

 

VitroGel® Cell Recovery Solution is a ready-to-use, enzyme-free solution to harvest 2D or 3D cultured cells from hydrogel fast and safely. The solution is compatible with VitroGel® hydrogel system and can recover cells from VitroGel in 15 minutes.

VitroGel Cell Recovery Solution is room temperature stable, has a neutral pH and works at 37°C operating temperature. The solution can maintain high cell viability during the recovery process. Cells can be sub-culture in both 2D and 3D culture after recovery.

The VitroGel Cell Recovery Solution can be used before or after the fixation and stained preparation of hydrogel specimens to ensure high quality of downstream data analysis.

Specifications

 

Formulation	Enzyme-free
Use	Harvest cells from VitroGel hydrogel while maintaining high cell viability. Use before or after sample fixation and stained preparation for imaging or downstream data analysis
Processing Time	15-20 min
Downstream	Recovered cells can be sub-culture in both 2D and 3D culture
pH	Neutral
Storage	Ambient Temperature (15-30°C)
Stability	30 months from date of manufacture
Size	100 mL

Product Documentation

• Cell-Recovery-Solution – Technical Data Sheet

Data and References

    

 Fiure 1. Fast hydrogel dissolved in VitroGel Cell Recovery Solution.

A. Hydrogel before adding to recovery solution; B-F. Time 0 to 15 min after adding hydrogel to recovery solution (at 37 °C, 20 rpm).

 

• Figure 2 The VitroGel Cell Recovery Solution maintain high cell viability

A. Cell viability of OP9, U87-MG and PANC-1 cells after adding to recovery solution at time 0, 15, 30, 60 and 120 min. Cells maintain over 95% cell viability after suspending in VitroGel cell recovery solution for 2 hrs; B. PANC-1 cells growth on 2D well plate before transfer to cell recovery solution; C. PANC-1 cells suspended in cell recovery solution for 24 hours then re-culture on 2D well plate for 5 days. Cells has been successful re-culture after suspend in cell recovery solution for 24 hours.

• Figure 3.

Cell viability of 3D cultured PANC-1 cells after recovering from hydrogel. (Method 1: add whole gel into cell recovery solution. Method 2: using pipette to break gel into small piece before adding into cell recovery solution. Average: the average cell viability of method 1 and method 2.)

• Figure 4. Cells can be sub-culture in both 2D and 3D culture after recovery. A.

PANC-1 cells growth on 3D hydrogel before harvested by VitroGel cell recovery solution; B. PANC-1 cells have been harvested from 3D hydrogel by using VitroGel cell recovery solution and subculture on the surface of hydrogel (day 2); C. PANC-1 cells have been harvested from 3D hydrogel by using the cell recovery solution and 3D subculture in the hydrogel system again (day 2).