0.2%明胶溶液
产品名称: 0.2%明胶溶液
英文名称: GSN
产品编号: 0423
产品价格: 0
产品产地: 美国ScienCell
品牌商标: ScienCell
更新时间: null
使用范围: null
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0.2%明胶溶液 货号0423
产品介绍
Gelatin is a heterogeneous mixture of water-soluble proteins of high-average molecular weights derived from collagen. 0.2% Gelatin Solution (GSN) is used to coat the surface of culture vessels to promote cell attachment. It is recommended for the culture of differentiated hESC cells [1, 2], certain primary and immortalized cell types.
Concentration
Sterile 0.2% gelatin from porcine skin in cell culture grade water.
Storage Conditions
Product is stable for 2 years and remains unchanged from the date of receipt when stored at 2 - 8° C. Keep sterile.
Applications
Substrate for cell culture adhesion. Optimal conditions for attachment must be determined for each cell line and application. Recommended concentration for normal human cell attachment is 100-200 μg/cm2.
Coating Procedure
The recommended coating concentration is 0.2% but may need optimization depending on cell type.
A. The following tables are a guide for the suggested volumes required per flask or plate well:
Flask |
0.2% Gelatin (ml) |
T-25 |
2.5 |
T-75 |
7.5 |
T-175 |
13 |