pET44c载体基本信息
出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列:  3' 测序引物:3' 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences (Novagen)
pET44c, pET 44c
大肠杆菌蛋白表达
高
多克隆位点，限制性内切酶
7309 bp
T7
T7: 5'-TAATACGACTCACTATAGGG-3'
ColiDOWN
ColiDOWN: 5'-TTCACTTCTGAGTTCGGCATG-3'
C-HSV, N-His, C-His, N-Thrombin, N-Nus, N-EK
Ampicillin
Both Nterm and Cterm His tags; Nterm thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS.
71124-3
瞬时表达 Transient
组成型 Constitutive
非病毒
pET44c载体质粒图谱和多克隆位点信息


Feature Name 
Start 
End 
 
T7_Terminal_primer
69
87
rrnB_T2_terminator
168
141
rrnB_T1_terminator
343
300
rrnB_terminator
466
309
EK
693
679
S15
789
745
6xHIS
2340
2323
T7_transl_en_RBS
2377
2361
lacO
2422
2395
T7_promoter
2440
2422
tet (300 - 563)
2476
2739
pBRrevBam_primer
2547
2528
lacI
2822
3913
ROP
4485
4676
pGEX_3_primer
4692
4670
pBR322_origin
5710
5091
AmpR_promoter
5797
5825
Ampicillin
5868
6728
ORF 
Start 
End 
 
ORF frame 1
427
2130
ORF frame 2
2352
499
ORF frame 1
2323
3081
ORF frame 2
2954
3913
ORF frame 3
5868
6728
Enzyme Name 
Cut
PacI
503
XhoI
530
PmlI
576
NotI
592
EagI
592
HindIII
599
KpnI
624
SalI
626
PstI
639
AscI
641
EcoRI
654
BamHI
660
SacI
672
SmaI
719
XmaI
717
SacII
795
SpeI
TUNEology 波长可调检测卡盒-->