T淋巴细胞激活蛋白抗体-抗体-抗体-生物在线
上海雅吉生物科技有限公司
T淋巴细胞激活蛋白抗体

T淋巴细胞激活蛋白抗体

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产品名称： T淋巴细胞激活蛋白抗体

英文名称： Sca1

产品编号： 3752

产品价格： null

产品产地： 上海

品牌商标： 雅吉

更新时间： null

使用范围： ELISA IHC-P IHC-F Flow-Cyt IF

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中文名称 T淋巴细胞激活蛋白抗体
别    名 Lymphocyte antigen 6A-2/6E-1; Ly-6A.2/Ly-6E.1; Stem cell antigen 1; Sca1 / Ly6A/E; SCA-1; T-cell-activating protein; TAP; Ly6al; Ly6a; LY6A_MOUSE.  
研究领域 细胞生物  免疫学  细胞膜受体  细胞表面分子  t-淋巴细胞  b-淋巴细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2μg /Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 9kDa
细胞定位 细胞膜 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from mouse Sca1/Ly6A/E:15-110/134 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
产品介绍 Sca1, also known as Ly6A/E, is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly6.1 and Ly6.2 allotypes. In the periphery, Sca1 exhibits a pattern of expression which is based on differences between the two allotypes. Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Sca1+ resting peripheral lymphocytes, whereas Ly6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Sca1+ lymphocytes. The expression of Sca1 is dramatically upregulated in all strains upon cellular activation.

Function:
T-cell activation.

Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity:
Widely expressed.

Post-translational modifications:
O-glycosylated. Not N-glycosylated.
Not phosphorylated.

Similarity:
Contains 1 UPAR/Ly6 domain.

SWISS:
P05533

Gene ID:
110454

Database links:

Entrez Gene: 110454 Mouse

SwissProt: P05533 Mouse

Unigene: 425949 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
产品图片 Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sca1 Polyclonal Antibody, Unconjugated(bs-3752R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sca1 Polyclonal Antibody, Unconjugated(bs-3752R) 1:500, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Tissue/cell: mouse thymus tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Sca1 Polyclonal Antibody, Unconjugated(bs-3752R) 1:500, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Blank control: Mouse Thymus Cells(blue).
Primary Antibody: Rabbit Anti-Sca1/FITC Conjugated antibody (bs-3752R/FITC), Dilution: 2μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.