pet26b载体基本信息
出品公司:别名:质粒类型:表达水平:克隆方法:载体大小:5' 测序引物:5' 测序引物序列:  载体标签:载体抗性:备注:产品目录号:稳定性:组成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET26b, pet-26b(+), pET 26b(+)
大肠杆菌蛋白表达
高
多克隆位点，限制性内切酶
5360bp
T7
5'-TAATACGACTCACTATAGGG-3'
C-His
卡那(Kanamycin)
含有pelB信号肽，蛋白表达时，能够将目的基因定位于细胞周质。
69862-3
瞬时表达 Transient
组成型 Constitutive
非病毒
pet26b载体质粒图谱和多克隆位点信息


pet26b多克隆位点信息：



Feature Name 
Start 
End
T7_terminator
129
1
T7_Terminal_primer
69
87
T7_transl_en_RBS
314
298
lacO
359
332
T7_promoter
377
359
tet (300 - 563)
409
672
pBRrevBam_primer
480
461
lacI
755
1846
tet (576 - 851)
1905
2180
ROP
2655
2846
pGEX_3_primer
2862
2840
pBR322_origin
3880
3261
KanR2
3986
4801

ORF 
Start 
End
ORF frame 1
514
1014
ORF frame 2
887
1846
ORF frame 2
3986
4801

Enzyme Name 
Cut
XhoI
158
NotI
166
EagI
166
HindIII
173
SalI
179
SacI
190
EcoRI
192
BamHI
198
NcoI
220
MscI
225
NdeI
288
XbaI
326
BglII
392
BclI
1128
ApaI
1325
HpaI
1620
FspI
2196
NruI
4074
ClaI
4108
SmaI
4291
XmaI
4289
pet26b载体简介
The pET-26b(+) vector (Cat. No. 69862-3) carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
pet26b载体序列
LOCUS