Overview

Product name
NAD/NADH Assay Kit


Assay type
Quantitative


Product overview
Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. The NAD/NADH Assay Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.


Notes
NADH is a sensitive molecule and can easily be used by different enzymes so it is recommended not to leave the lysates longer. The Cell/ tissue extract should also be kept cold. To avoid interference from other enzymes we recommend deproteinate the samples immediately upon harvesting using 10kD spin column (ab93349).


Tested applications
Functional Studiesmore details

Properties

Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C. Store in the dark. Avoid repeated freeze / thaw cycles.



    

        

            
Components
            
Identifier
            
100 tests
        
    
    

        

            
NAD Cycling Buffer
            
NM
            
1 x 15ml
        
        

            
NAD Cycling Enzyme Mix
            
Green
            
1 vial
        
        

            
NADH Developer
            
Purple
            
1 vial
        
        

            
NADH Standard (MW:763)
            
Yellow
            
1 x 152.6µg
        
        

            
NADH/NAD Extraction Buffer
            
NM
            
1 x 50ml
        
        

            
Stop Solution
            
Red
            
1 x 1.2ml
        
    


http://www.abcam.com/NAD-NADH-Assay-Kit-ab65348.html