MSCV-CMV-DsRed-IRES-d24EGFP
(Plasmid #41944)

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PURPOSE

(Empty Backbone) Retroviral expression of DsRed in mammalian cells
DEPOSITING LAB

Stephen Elledge
PUBLICATION

Yen et al Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489.
PRICE

$65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

Vector backbone
MSCV
(Search Vector Database)
Backbone size (bp)7556
Vector type
Mammalian Expression, Retroviral
Selectable markers
Puromycin ; IRES d24EGFP

GROWTH IN BACTERIA

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
ccdB Survival
Copy number
High Copy

SEQUENCE INFORMATION

Depositor Sequences

None.
Addgene Sequences

Partial (3)

Full plasmid sequence is available only if provided by the depositing laboratory.

GENE/INSERT

Gene/Insert name
DsRed
PromoterTRE

CLONING INFORMATION

Cloning methodGateway Cloning
5′ sequencing primerpLXSN-5
3′ sequencing primerMSCV-rev

(Common Sequencing Primers)

RESOURCE INFORMATION

Terms and Licenses

DEPOSITOR COMMENTS

24 hour half life EGFP

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

MSCV-CMV-DsRed-IRES-d24EGFP was a gift from Stephen Elledge (Addgene plasmid # 41944)
For your References section:

Global protein stability profiling in mammalian cells. Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ. Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489. 10.1126/science.1160489 PubMed 18988847

MSCV-CMV-DsRed-IRES-d24EGFP逆病毒载体质粒图谱序列抗性

MSCV-CMV-DsRed-IRES-d24EGFP (Plasmid #41944)