pBIN19 map and sequence:

>pBin19, 11777 bp

ID   PBIN19     preliminary; circular DNA; SYN; 11777 BP.

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AC   U09365; U12540; IG1039; X04996; X04995; V00087; J01541; M61152;

AC   M38354; S54410; M14641; ATCC37327;

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DT   01-FEB-1993 (Rel. 7, Created)

DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)

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DE   Higher plants/E.coli plasmid vector pBin19 - incomplete.

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KW   cloning vector.

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OS   Cloning vector

OC   Artificial sequences; Cloning vehicles.

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RN   [1]

RC   plasmid from linker & pBR325, kan gene

RC   pUC9-R1 from pUC9

RC   pUC9-nopneodelta from pUC9-R1 & plasmid

RC   [pAT21-1 from pAT series]

RC   plasmid2 from pAT21-1 & pRK252

RC   pBIN6 from plasmid2 & pTiT37

RC   pBIN19 from pBIN6 & pTiT37 & M13mp19

RA   Bevan M.W.;

RT   "Binary Agrobacterium vectors for plant transformation";

RL   Nucleic Acids Res. 12:8711-8721(1984).

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RN   [2]

RC   pOTY8 from pJDB207 & pTiAch5

RC   pAL1050 from pOTY8 & R772, IS70

RC   pAL4404 from pTiT37

RA   Hoekema A., Hirsch P.R., Hooykaas P.J., Schilperoort R.A.;

RT   "A binary plant vector strategy based on separation of vir- and

RT   T-region of the Agrobacterium tumefaciens Ti-plasmid";

RL   Nature 303:179-180(1983).

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RN   [3]

RP   1-3213

RC   pBIN19 from pBIN6 & pTiT37 & M13mp19

RA   Wei W., Dekker B., Lindsey K.;

RT   "T-DNA sequence of plant transformation vector pBin19";

RL   Unpublished (1994).

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RN   [4]

RP   1-3213

RC   pBIN19

RA   Wei W.;

RT   ;

RL   Submitted (22-JUL-1994) by:

RL   Wei W., Botany, University of Leicester, University Road,

RL   Leicester, LE1 7RH, UK.

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RN   [5]

RP   1-11777

RC   pBIN19 from pBIN6 & pTiT37 & M13mp19

RA   Frisch D.A., Hardin S.H., Harris-Haller L., Yokubaitis N.T.,

RA   Thomas T.L., Hall T.C.;

RT   "Fortuitous ori and other sequences in Bin 19";

RL   Unpublished (1994).

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RN   [6]

RP   1-11777

RC   pBIN19

RA   Frisch D.A.;

RT   ;

RL   Submitted (04-MAY-1994) by:

RL   Frisch D.A., Biology, Texas A&M University, College Station,

RL   TX 77843-3155, USA.

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CC   Restriction digests of the clone give the following sizes (kb):

CC   EcoRI--10.5. (ATCC staff)

CC   A vector for efficient transformation of higher plants in a binary

CC   plasmid system with pAL440. (personal communication)

CC   Medium is 1065 LB medium.

CC   NCBI gi: 520486

CC   NCBI gi: 529322

CC   NM (pBin19)

CC   CM (no)

CC   NA (ds-DNA)

CC   TP (circular)

CC   ST ()

CC   TY (plasmid)

CC   SP (CLONTECH)(ATCC)

CC   HO (Agrobacterium tumefaciens)(E.coli HB101)(higher plants)(E.coli)

CC   CP ()

CC   FN (cloning)(reporter)

CC   SE ()

CC   PA ()

CC   BR ()

CC   OF (pBI101)(pBI101.2)(pBI101.3)

CC   OR ()

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FH   Key             Location/Qualifiers

FH

FT   misc_feature    0..0

FT                   /note="1. pBR325 BamHI 5994bp 376..376

FT                   2. Tn5 BglII-BamHI 1541bp 1516..3057, kan/neo gene

FT                   -> plasmid 7535bp

FT                   1. plasmid Sau3A/MboI-Sau3A/MboI 330bp 1521..1851,

FT                   \ 5 kan/neo gene

FT                   Klenow:Klenow

FT                   BglII linker 6bp agatct:BglII linker 6bp agatct

FT                   BglII-PstI 216bp 1516..1732, 5 kan/neo gene

FT                   2. plasmid PstI-BamHI 402bp 2655..3057, 3 kan/neo

FT                   -> plasmid2 1150bp

FT                   1. pUC9 remove HindIII-EcoRI 30bp 234..264, MCS/2635bp

FT                   2. pTiT37 HindIII-EcoRI, nos promoter/terminator

FT                   -> plasmid3

FT                   1. plasmid2 BglII-BamHI 1150bp, kan/neo gene

FT                   2. plasmid3 BamHI, between cap and polyA

FT                   -> plasmid4

FT                   1. plasmid4 BamHI, between kan and polyA

FT                   fill in

FT                   -> plasmid5

FT                   1. plasmid5 EcoRI-HindIII, nos prom/kan gene/nos polyA

FT                   2. pTiT37 HindIII-HindIII 3200bp, nos gene/right T-DNA

FT                   -> plasmid6

FT                   1. pTiT7 KpnI-KpnI 3800bp, left T-DNA border

FT                   Klenow:Klenow

FT                   2. plasmid6 EcoRI-HindIII, nos prom/kan/polyA/nos/T

FT                   Klenow:

FT                   -> plasmid7 [T/nos prom/kan/polyA/nos/T]

FT                   1. plasmid7 remove SstII-SstII, nos gene