Media Reference Books, Software and Video Cassettes

Technical Information

Many chromatographers have come to rely on our extensive experience. In addition to our widely respected technical data files and manuals, we offer basic educational software, videos, and printed materials covering a variety of chromatography techniques. Ordering information shows educational materials that are available for order. Note that GE Healthcare method handbooks are available in PDF format for download at www.gelifesciences.com/handbooks.

Protein Sample Preparation Handbook
The overall goal of sample preparation is to feed the analytical technique of choice with protein subsamples of a quality that maximizes the chances for a successful analysis. This handbook is divided into five chapters, starting with an overview of protein sample preparation, followed by three chapters based on a conceptual, high-level workflow for protein sample preparation and analysis. The handbook wraps up with a chapter that focuses on parallel processing and screening strategies in recombinant protein and monoclonal antibody production workflows.

Nucleic Acid Sample Preparation for Downstream Analyses: Principles and Methods
Poor sample preparation can lead to suboptimal results in downstream applications. The goal of this handbook is to provide useful information to help make this very important starting point as prescriptive and efficient as possible. This handbook provides guidance, hints, and tips and is intended for novice and experienced researchers to aid in “getting it right from the start” when preparing nucleic acids for downstream applications.

Affinity Chromatography: Principles and Methods
Advice and practical information about affinity chromatography and affinity products, including many applications showing the ease of use of this technique.

Antibody Purification
Presents the most effective and frequently used strategies for sample preparation and purification of the many different forms of antibodies and antibody fragments used in the laboratory.

Purifying Challenging Proteins: Principles and Methods
This handbook is intended for students and experienced researchers with an interest in the isolation of integral membrane proteins, multiprotein complexes, or in refolding proteins from inclusion bodies. The aim of the handbook is to present tools, strategies, and solutions available to meet the purification challenges associated with these three classes of proteins.

Column Packing - The Movie
CD-ROM
The success of a protein purification experiment can depend on how well you pack your column. Correct column packing is therefore essential.
This CD-ROM contains instructional movies that demonstrate good packing techniques for gel filtration and adsorptive chromatography columns. You will also learn how to check the efficiency of the column packing. For Windows and Macintosh.

Gel Filtration, Principles and Methods
Since the introduction of Sephadex™ forty years ago, gel filtration has occupied a key role in the purification of thousands of biological macromolecules. Its enduring value depends partially on the special nature of these biomolecules and the reliability and simplicity of gel filtration as a separation technique.

Hydrophobic Interaction and Reversed Phase Chromatography
This handbook describes both theoretical and practical aspects of the technique, the media available and how to select them, together with application examples and detailed instructions for the most commonly performed procedures. Practical information, with many tips and hints drawn from over 40 years of experience in chromatography purification, guides beginners and experts toward obtaining the best possible results from the latest chromatography media.

Ion Exchange Chromatography & Chromatofocusing: Principles and Methods
This handbook contains the latest information on the theoretical and practical aspects of ion exchange and chromatofocusing techniques, the prepacked columns and media available, and how to select them. The handbook provides many application examples, practical information together with hints and tips on improving ion exchange chromatography and chromatofocusing protocols.

Strategies for Protein Purification Handbook
Strategies for Protein Purification is focused on chromatographic purification of proteins at research scale. This handbook describes how to design a protein purification procedure, how to select and combine chromatography methods and purification formats, and how to monitor and evaluate protein purification

Protein Purification: Principles, High Resolution Methods, and Applications, volume 54 edited by J.-C. Janson.
This standard work in the field of protein purification covers a broad range of techniques, both in chromatography and electrophoresis. It provides a comprehensive background for anyone seriously moving into the area. Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in biosciences has dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters that have been completely rewritten.

Recombinant Protein Purification: Principles and Methods
Includes the most recent methodologies in the rapidly developing field of purification of histidine-tagged and GST-tagged proteins.
Application examples illustrate recovery and purification of recombinant proteins from different sources and hosts

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