Poly FLAG Peptide lyophilized powder,中国库存，Biotool美国品牌。
产品名称： Poly FLAG Peptide lyophilized powder,中国库存，Biotool美国品牌。
英文名称： Poly FLAG Peptide lyophilized powder
产品编号： B23111
产品价格： 0
产品产地： 美国
品牌商标： Biotool
更新时间： null
使用范围： null
Poly FLAG Peptide lyophilized powder
Price Comparison
| Brand | 4 mg | 25 mg |
|---|---|---|
| Biotool | 99.00 USD | 399.00 USD |
| Sigma | 299.00 USD | 1375.00 USD |
Properties
| Appearance (Color) | White |
|---|---|
| Appearance (Form) | Powder |
| Form | Lyophilized Powder |
| Purity by HPLC-MS | > 95 % |
| Concentration | (Recommended working concentration is 100-400 μg/ml for elute FLAG fusion proteins from the Biotool Anti-DYKDDDDK (FLAG) affinity gel. |
| Shipped in | Blue ice |
| Storage Temperature | 2-8°C |
Description
The Poly DYKDDDDK (FLAG) Peptide lyophilized powder was synthesized by 23 amino acid residues with molecular weight 2,864 Da. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).
Application
For use in competitive elution of DYKDDDDK (FLAG) fusion proteins from the ANTI-FLAGmonoclonal antibody in solution or bound to agarose on the Biotool Anti-DYKDDDDK (FLAG) affinity gel.
Attention
1. Read the User Manual carefully before the first use;
2. Avoid freezing, drying and high-speed centrifugation during use and storage of beads;
3. This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Procedure
Peptide Elution of DYKDDDDK (FLAG) Fusion Protein from Biotool Anti-DYKDDDDK (FLAG) affinity gel
1. Thoroughly suspend the Anti-Flag Affinity Gel in the vial, for a uniform suspension of the resin. Quickly transfer 10μl of the gel suspension (about 5μl of packed gel volume) to a fresh tube.
2. Add 0.6ml TBS. Thoroughly suspend the Anti-Flag Affinity Gel by pipetting. Centrifuge the resin at10000 rpm for 30 secondsand carefully remove the supernatant. Be sure to remove all of the wash buffer without discarding the resin. Repeat 3-4 times.
3. Add 500μl of cell periplasmic extracts to the washed resin.
4. Gently agitate samples for 2 hours at 4℃.
5. Centrifuge the resin for 30 seconds at 4500 rpm. Transfer the supernatants to a fresh tube.
6. Wash the resin with 0.5ml TBS until the OD280 of the supernatant reads＜0.05.
7. Elution of DYKDDDDK (FLAG) Fusion Protein by Competition with Poly DYKDDDDK (FLAG) Peptide. Elute the bound DYKDDDDK (FLAG) Fusion Proteinby competitive elution with five one-column volume aliquots of a solution containing 100-400ug/mLPoly DYKDDDDK (FLAG) Peptide in TBS.
8. Recycling the Biotool Anti-DYKDDDDK (FLAG) affinity gel
Poly DYKDDDDK (FLAG) Peptidemay not elute all of the DYKDDDDK (FLAG) Fusion Protein bound to Biotool Anti-DYKDDDDK (FLAG) affinity gel. It is recommended the Biotool Anti-DYKDDDDK (FLAG) Affinity gel be regenerated immediately after use by washing with three 5 ml aliquots of 0.1 M glycine HCI, pH 3.5. The gel should be immediately re-equilibrated in TBS until the effluent is at neutral pH.
Note: Do not leave the Biotool Anti-DYKDDDDK (FLAG) Affinity gel in glycine HCl for longer than 20 minutes.
9. Storing the Biotool Anti-DYKDDDDK (FLAG) Affinity gel
Wash the Biotool Anti-DYKDDDDK (FLAG) Affinity gel three times with 5 ml of 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide. then add another 5 ml of 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azideand store at -20°C without draining.
Storage & stability
Store the product at 2-8°C.
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