氧自由基作用于脂质的不饱和脂肪酸，生成过氧化脂质；后者逐渐分解为一系列复杂的化合物，其中包括MDA。通过检测MDA的水平即可检测脂质氧化的水平。
MDA与硫代巴比妥酸(thiobarbituric acid，TBA)缩合，生成红色产物，在532nm有最大吸收峰，进行比色后可估测样品中过氧化脂质的含量；同时测定600nm下的吸光度，利用532nm与600nm下的吸光度的差值计算MDA的含量。
试验中所需的仪器和试剂：
可见分光光度计、水浴锅、台式离心机、可调式移液器、1ml玻璃比色皿、研钵、冰和蒸馏水。
产品内容：
提取液：液体60mL×1瓶，4℃保存；
试剂一：液体30mL×1瓶，4℃保存；
注意：临用前注意试剂一是否完全溶解，如未溶解，可以70℃加热，并震荡以促进溶解。

相关文献：

《Protective effect of agmatine against hyperoxia-induced acute lung injury via regulating lncRNA gadd7》 作者:Guoyue Liu,Hong Mei,Miao Chen,Song Qin,Kang Li,Wei Zhang,Tao Chen 期刊:Biochemical and Biophysical Research Communications 影响因子:2.705 PMID:31196629
《Ocular toxicity of reduced graphene oxide or graphene oxide exposure in mouse eyes》 作者:Wenzhen An,Ying Zhang,Xuan Zhang,Kang Li,Yujun Kang,Shahnaz Akhtar, Xueli Sha,Lan Gao 期刊:Experimental Eye Research 影响因子:2.998 PMID:29803558
《Knockdown of lncRNA AK139328 alleviates myocardial ischaemia/reperfusion injury in diabetic mice via modulating miR‐204‐4p and inhibiting autophagy》 作者:Si‐yang Yu,  Bo Dong , Zhen‐fei Fang,  Xin‐qun Hu,  Liang Tang , Sheng‐hua Zhou 期刊:Journal of Cellular and Molecular Medicine  影响因子:4.658 PMID:30047214
《Effects of Quercetin on Proliferation and H2O2-Induced Apoptosis of Intestinal Porcine Enterocyte Cells》 作者:Zhigang Chen , Qiaoling Yuan , Guangren Xu , Huiyu Chen , Hongyu Lei  and Jianming Su 期刊:Molecules 影响因子:3.06 PMID:
《Hydrogen-water ameliorates radiation-induced gastrointestinal toxicity via MyD88’s effects on the gut microbiota》 作者:Hui-wen Xiao, Yuan Li, Dan Luo, Jia-li Dong, Li-xin Zhou, Shu-yi Zhao, Qi-sheng Zheng, Hai-chao Wang, Ming Cui & Sai-jun Fan 期刊:experimental and molecular medicine 影响因子:4.743 PMID:29371696
《Toxic effect of microcystin-LR on blood vessel development》 作者:Qilong Wang, Guosheng Xiao, Guoliang Chen, Huihui Du, Linping Wang,Dongqin Guo & Tingzhang Hu 期刊:Toxicological & Environmental Chemistry 影响因子:3.547 PMID:28189720
《A C2H2 zinc-finger protein OsZFP213 interacts with OsMAPK3 to enhance salt tolerance in rice》 作者:Zeyong Zhang,Huanhuan Liu,Ce Sun,Qibin Ma,Huaiyu Bu,Kang Chong,Yunyuan Xu 期刊:Journal of Plant Physiology 影响因子:2.825 PMID:30055519
《Physiological and transcriptomic analysis revealed the involvement of crucial factors in heat stress response of Rhododendron hainanense》 作者:Ying Zhao,Wengang Yu,Xiangyu Hu,Youhai Shi,Yu Liu,Yunfang Zhong,Peng Wang,Shuya Deng,Jun Niu,Xudong Yu 期刊:Gene 影响因子:2.638 PMID:29604462
《Identification, Expression, and Functional Analysis of the Group IId WRKY Subfamily in Upland Cotton (Gossypium hirsutum L.)》 作者:Lijiao Gu1, Hantao Wang, Hengling Wei, Huiru Sun, Libei Li, Pengyun Chen, Mohammed Elasad, Zhengzheng Su, Chi Zhang, Liang Ma, Congcong Wang and Shuxun Yu 期刊:Frontier in Immunology 影响因子:4.259 PMID:
《Environmental stress stability of pectin-stabilized resveratrol liposomes with different degree of esterification》 作者:Ping Shao,Pei Wang,Ben Niu,Ji Kang 期刊:International Journal of Biological Macromolecules 影响因子:4.784 PMID:30036624
《Regulation of TBBPA-induced oxidative stress on mitochondrial apoptosis in L02?cells through the Nrf2 signaling pathway》 作者:YunchaoZhanga1XiaoliWanga1ChaoChenaJingAnbYuShangbHuiLiabHubinXiaaJunYuaChenWangaYongdiLiuaShuGuo 期刊:Chemosphere 影响因子:5.108 PMID:30951941
《Amelioration of nonalcoholic fatty liver disease by swertiamarin in fructose-fed mice》 作者:YangYangabLiJingabWeiCongabHeYingabCaoYixinabZhangYongminabcSunWenjiabQiaoBolingabHeJiao 期刊:Phytomedicine 影响因子:4.18 PMID:31005808
《Curcumin attenuates hypoxia/reoxygenation?induced cardiomyocyte injury by downregulating Notch signaling》 作者:Peng Zhu Manli Yang Hao He Zhibin Kuang Mu Liang Anxiao Lin Song Liang Qiyun Wen Zhiqin Cheng Chaofeng Sun 期刊:Mol Med Rep 影响因子:1.851 PMID:31257466