鞘氨醇激酶1(SPHK1)重组蛋白说明书

Recombinant Sphingosine Kinase 1 (SPHK1) 

[PROPERTIES]

Source: Prokaryotic expression

Host: E.coli

Residues: Leu148~Leu398

Tags: N-terminal His Tag

Subcellular Location: Nucleus, Cytoplasm

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose

and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.9

Predicted Molecular Mass: 29.2kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions

[USAGE]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[STORAGE AND STABILITY]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[SEQUENCE]

[IDENTIFICATION]

[IMPORTANT NOTE]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.
本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用