蛋白单克隆即用型免疫组化试剂盒
| 英文名称 | S100B Ready-To-Use IHC Kit | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 中文名称 | 蛋白单克隆即用型免疫组化试剂盒 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 别 名 | NEF; Protein S100 B; Protein S100-B; S 100 calcium binding protein beta chain; S 100 protein beta chain; S 100 protein beta subunit; S-100 protein beta chain; S-100 protein subunit beta; S100; S100 calcium binding protein B; S100 calcium binding protein beta (neural); S100 calcium binding protein beta chain; S100 calcium-binding protein B; S100 protein beta chain; S100B_HUMAN; S100beta; S100 β; S100-β; S100β. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 种 属 | Human, Mouse, Rat | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 应用范围 | Immunohistochemistry | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 适用样品基质 | PFPE tissue | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 描述 | IHC0110 is a ready-to-use IHC kit for staining of S100B. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 保存条件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| 产品介绍 |
产品组分及规格
背景 S100B is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells and are involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes.
石蜡包埋组织的免疫组织化学方案 1. 脱蜡水化 石蜡切片置于新鲜二甲苯中浸泡脱蜡3次，每次15 min；依次置于不同浓度（100%、95%、90%、80%、70%）乙醇浸泡各5 min，再置于蒸馏水洗涤5 min，重复3次。 2. 抗原修复 沸水浴修复：将100×抗原修复缓冲液（试剂2）用蒸馏水稀释成1×抗原修复缓冲液，放入修复盒中并提前加热至95-100℃（注意盖好以防液体蒸发），然后将切片放入修复盒中，在沸水浴环境中保持外沸状态15 min，室温自然冷却；用PBS缓冲液（试剂1，将干粉溶解在2L蒸馏水中）清洗5 min，重复3次。 3. 阻断内源性过氧化物酶 用吸水纸吸去玻片上多余的液体，用免疫组化笔在组织周围画圈，加入2-4滴内源性过氧化物酶阻断剂（试剂3），室温下置于湿盒中孵育15 min，用PBS洗涤5 min，重复3次。 4. 血清封闭 用吸水纸吸去玻片上多余的液体，加入2-4滴封闭工作液（试剂4），置于湿盒内37℃封闭20 min，以减少非特异性染色。 5. 一抗孵育 用吸水纸吸去玻片上多余的液体，加入2-4滴S100B
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