原肌球调节蛋白1(TMOD1)重组蛋白说明书

Recombinant Tropomodulin 1 (TMOD1)   

[ DESCRIPTION ]

 Protein Names: Tropomodulin 1

 Synonyms:TMOD1, D9S57E, TMOD

Species: Human

Size: 100µg

Source:Escherichia coli-derived

Subcellular Location :Cytoplasm, cytoskeleton.

[ PROPERTIES ]

Residues: : Pro39~His138 (Accession # P28289), withN-terminal His-Tag

Grade & Purity:  >95%, 15kDa as determined bySDS-PAGE reducing conditions

 Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

 Endotoxin Level: : <1.0 EU per 1μg (determined bythe LAL method).

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Molecular Mass: 12.7kDa

Predicted isoelectric point: 5.6

[ PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37ºC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below. 

MGHHHHHHSGSEF- PD NALLPAGLRQ KDQTTKAPTG PFKREELLDH LEKQAKEFKDREDLVPYTGE KRGKVWVPKQ KPLDPVLESV TLEPELEEAL ANASDAELCD IAAILGMH

本产品仅供科研使用。不能用于人和动物治疗等其它临床诊断使用