链霉亲和素-分析方法-资讯-生物在线
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链霉亲和素

作者：上海信裕生物科技有限公司 2023-04-25T00:00 (访问量:385)

 链霉亲和素

英文名称 Streptavidin
中文名称 链霉亲和素
别    名 SA V1; SA V2; Streptavidin V1; Streptavidin V2; SA. ;Streptavidin  链亲和素; Streptavidin 链霉亲和素;
Specific References  (17)     |     bs-0437P has been referenced in 17 publications.
111 [IF=5.01] Dong, Peipei, et al. "Ultrathin Gold-Shell Coated Silver Nanoparticles onto a Glass Platform for Improvement of Plasmonic Sensors." ACS Applied Materials & Interfaces (2013).  Other ;  
 
111 [IF=4.57] Pu, Caixiu, et al. "Ultrasound-Mediated Destruction of LHRHa Targeted and Paclitaxel Loaded Lipid Microbubbles for the Treatment of Intraperitoneal Ovarian Cancer Xenografts." Molecular pharmaceutics (2013).  other ;  Human.  222
 
111 [IF=4.57] Liu, Hongxia, et al. "Ultrasound-Mediated Destruction of LHRHa Targeted and Paclitaxel Loaded Lipid Microbubbles Induces Proliferation Inhibition and Apoptosis in Ovarian Cancer Cells." Molecular Pharmaceutics (2013).  other ;  Biotin.  222
 
111 [IF=3.55] Huang, Mo, et al. "Solidified liquid layer model makes quartz crystal microbalance a convenient molecular ruler." Colloids and Surfaces B: Biointerfaces 85.1 (2011): 92-96.  Other ;  
 
111 [IF=3.54] Chen, Feifei, et al. "Direct growth of coupled gold nanoparticles on indium tin oxide substrate and construction of biosensor based on localized surface plasmon resonance." Sensors and Actuators B: Chemical (2013).  other ;  Biotin.  222
 
111 [IF=5.44] Zhang, Juan, et al. "Amplified amperometric aptasensor for selective detection of protein using catalase-functional DNA-PtNPs dendrimer as a synergetic signal amplification label." Biosensors and Bioelectronics (2014).  other ;  
 
111 [IF=3.43] Jiang, Yixuan, et al. "Sensitive aptamer-based fluorescence polarization assay for mercury (II) ions and cysteine using silver nanoparticles as a signal amplifier." Microchimica Acta (2014): 1-8.  Other ;  
 
111 [IF=5.977] Jing Chen. et al. A novel method combining aptamer-Ag10NPs based microfluidic biochip with bright field imaging for detection of KPC-2-expressing bacteria. Anal Chim Acta. 2020 Oct;1132:20  Other ;  
 
111 [IF=3.375] Yun Liu. et al. Synergistic anti-tumor effect of anti-PD-L1 antibody cationic microbubbles for delivery of the miR-34a gene combined with ultrasound on cervical carcinoma. Am J Transl Res. 2021; 13(3): 988–1005  Other ;  
 
111 [IF=14.188] Xuehui Liu. et al. Stimuli-Mediated Specific Isolation of Exosomes from Blood Plasma for High-Throughput Profiling of Cancer Biomarkers. 2021 Dec 19  
 
111 [IF=5.221] Yang Guo . et al. Highly Sensitive Detection of Carcinoembryonic Antigen via an Electrochemical Platform Fabricated by AuNPs/Streptavidin/Reduced Graphene Oxide. FRONT CHEM. 2022; 10: 898924  
 
111 [IF=4.162] Houren Zhou. et al. “PFH/AGM-CBA/HSV-TK/LIPOSOME-Affibody”: Novel Targeted Nano Ultrasound Contrast Agents for Ultrasound Imaging and Inhibited the Growth of ErbB2-Overexpressing Gastric Cancer Cells. DRUG DES DEV THER. 2022; 16: 1515–1530  
 
111 [IF=8.008] Li Fu. et al. A General Route for Chemiluminescence of n-Type Au Nanocrystals. ANAL CHEM. 2022;94(24):8811–8817  IA ;  
111 [IF=6.911] Zhuoer Zeng. et al. Nonlinear hybridization chain reaction-based flow cytometric immunoassay for the detection of prostate specific antigen. ANAL CHIM ACTA. 2022 Aug;1220:340048  
111 [IF=10.334] Zhao Cui. et al. Chip-DSF: A rapid screening strategy for drug protein targets. PHARMACOL RES. 2022 Aug;182:106346  
 
111 [IF=6.14] Wenhao Li. et al. Biotinylated Au Nanoparticle-Based Artificial Antibody for Detection of Lysozyme by the Lateral Flow Immunoassay and Enzyme-Linked Immunosorbent Assay. ACS APPL NANO MATER. 2022;XXXX(XXX):XXX-XXX  Other ;  Other.  222
 
111 [IF=8.008] Yi Ma. et al. Flap Endonuclease-Induced Steric Hindrance Change Enables the Construction of Multiplex and Versatile Lateral Flow Strips for DNA Detection. ANAL CHEM. 2022;94(42):14725–14733  Other ;  Other.  222
 
 
理论分子量 12kDa
检测分子量 12/38/55 kDa
性    状 Lyophilized powder
浓    度 ＞0.5 mg/ml
物    种 Streptomyces
序    列 25-183/183
纯    度 >95% as determined by SDS-PAGE
纯化方法 AC
内毒素 Not analyzed
表达系统 N/A
活性 Yes
标签 Tag free
缓 冲 液 Lyophilized from 0.22 um filtered solution in PBS (pH=7.4) with 5 mM DTT. Normally 5% trehalose is added as protectant before Lyophilization.
保存条件 Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Streptavidin is a tetrameric protein composed of identic subunits. Each subunit binds one biotin molecule with a KD of ~1x10-15M. The preparation contains an N- and C-terminal shortened variant (core streptavidin) with improved properties concerning homogeneity, solubility, resistance towards proteolytic degradation and accessibility of the biotin binding pocket as compared to native streptavidin. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.



链霉亲和素（Streptavidin,SA）产品简介
链霉亲和素具有与亲和素(Avidin,AV)相似的生物学特性，如都能与4分子的生物素（Biotin）特异性结合，结合常数为1×10-15/M。同时，由于SA不带糖基、等电点低，在检测中具有比AV低的背景而大大提高了检测的灵敏性。
生物素与亲和素/链霉亲和素的结合具有高度特异性和稳定性，两者的亲和常数比抗原-抗体反应高10-100万倍，是目前已知强度最高的非共价作用，这使得它成为免疫学检测中的一个非常好信号放大的工具。广泛应用于酶联免疫反应、荧光分子标记免疫反应和分子杂交为原理的各种检测试剂盒的SA-biotin系统。但目前，国内所用SA均为价格昂贵的进口产品，因此研制和生产国产化的SA具有重要的现实意义和广阔的应用前景。
    链霉亲和素是与亲和素具有相似性质的一种蛋白质，都具有与生物素特异结合的能力，结合常数高达1015M。同时，由于链霉亲和素等电点比亲和素低，且不含糖基链，故在检测中的灵敏度和特异性都高于亲和素，建立在此基础上的生物素-链霉亲和素系统的应用比生物素-亲和素系统有更大优势。
    我公司生产用链霉亲合素-SA菌株及生产工艺均自国外原本带回。生产工艺稳定，保证质量，常年提供产品，确保供应。
我公司生产的SA单体经SDS-PAGE检验分子量为17.2KDa。经论证，完整的SA溶解性差、易于聚集，核心SA被蛋白酶水解掉了与活性无关的某些末端序列，一方面提高了结构的稳定性；另一方面由于减少了空间位阻，使SA更容易与生物素标记的大分子发生反应。
SA的纯化是用2-亚氨基生物素琼脂糖珠4B 凝胶(Sigma 产品)进行亲和层析纯化，同时以离子交换进行进一步的精细纯化。
SA分子量为58KDa,生物活性15.7U/mg，接近理论值（16.8U/mg）并进行了活性测定。
SA溶解与保存：我公司提供的SA产品，不含任何盐离子，用户可以根据自己的试验需要，使用不同的缓冲液进行溶解，溶解后应放与-20°C保存，避免反复冻溶。
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